A MATLAB Pipeline for Spatiotemporal Quantification of Monolayer Cell Migration

One-dimensional collective migration of a proliferating cell monolayer.

The importance of collective cellular migration during embryogenesis and tissue repair asks for a sound understanding of underlying principles and mechanisms. Here, we address recent in vitro experiments on cell monolayers, which show that the advancement of the leading edge relies on cell proliferation and protrusive activity at the tissue margin. Within a simple viscoelastic mechanical model ...

Quantification and regulation of cell migration.

Cell migration is essential for many physiological and pathological processes that include embryonic development, the immune response, wound healing, angiogenesis, and cancer metastasis. It is also important for emerging tissue engineering applications such as tissue reconstitution and the colonization of biomedical implants. By summarizing results from recent experimental and theoretical studi...

A computational pipeline for quantification of mouse myocardial stiffness parameters

The mouse is an important model for theoretical-experimental cardiac research, and biophysically based whole organ models of the mouse heart are now within reach. However, the passive material properties of mouse myocardium have not been much studied. We present an experimental setup and associated computational pipeline to quantify these stiffness properties. A mouse heart was excised and the ...

Spatiotemporal dynamics of microRNA during epithelial collective cell migration.

MicroRNAs (miRNAs) are small, noncoding RNAs variably involved in a wide variety of developmental and regenerative programs. Techniques for monitoring the spatiotemporal expression of miRNA in living cells are essential to elucidate the roles of miRNA during these complex regulatory processes. The small size, low abundance, sequence similarity, and degradation susceptibility of miRNAs, however,...

Propagation of goat pox virus on monolayer cell cultures